Phenotypic and molecular characterization of typhoidal and non-typhoidal Salmonella isolates from humans, animals and the environment in Ethiopia
PhD Candidate: Anteneh Habte
Institutions: Addis Ababa University and Armauer Hansen research Institute
Supervisors: Dr. Aklilu Feleke, Dr. Adane Mihret, Dr. Andargachew Mulu
Salmonella enterica and Salmonella bongori the two Gram negative, motile, facultative anaerobic rod-shaped species of salmonella belonging to the Enterobacteriaceae family. It resides in the digestive system and blood stream of humans and animals. WHO reported that 9 million people get sick and 110,000 people die from typhoid fever every year. Antimicrobial resistance (AMR) has been recognized as one of the major health threats to people and animals of global concern specially in east Africa and Ethiopia. Application of whole genome sequencing for surveillance enabled identification and characterization of the bacteria.
Objectives: To detect, identify and characterize Typhoidal and Non Typhoidal Salmonella Serovars from human, chicken, cattle and environmental samples in Ethiopia. Understand their population structure and clonal relationship
Methods: A cross-sectional study will be conducted from September 2024 to August 2027 in Addis Ababa, Adama, Assela and Hawassa cities of Ethiopia. 328 blood/stool samples from humans, 765 samples from chickens/cattle and 272 samples from food and water will be collected. The collected samples will be tested for the availability of salmonella and each isolated will be further tested for drug susceptibility and sequenced for further studies.
Expected outcomes: Provide evidence on the burden of typhoid fever and inform the actual contribution of animals and environment on Salmonella transmission, virulence and drug resistance in Ethiopia
Result dissemination plan: The findings of this study will be presented to Addis Ababa university and Armauer Hansen Research Institute community in a form of thesis. In addition, the data will be presented on appropriate national and international scientific conferences. Furthermore, manuscripts will be prepared and submitted for publication on peer-reviewed journals.